chemical composition and antibacterial properties of ocimum basilicum, salvia officinalis and trachyspermum ammi essential oils alone and in combination with nisin
نویسندگان
چکیده
background and objectives:plant essential oils are sometimes considered for use as antimicrobial agents in foods and medicines and they could be combined with other antimicrobial agents to strengthen the effect and/or reduce the required dose. this study was conducted to determine the chemical composition of the ocimum basilicum, salvia officinalis and trachyspermum ammi essential oils and evaluate their antibacterial efficiency, alone and in combination with nisin, against escherichia coli o 157 and staphylococcus aureus. methods: the chemical composition of three essential oils (ocimum basilicum, salvia officinalis and trachyspermum ammi) were determined by gas chromatography/mass spectrometry. further, their antibacterial properties and the synergistic effect of the combination of three essential oils and nisin were also assessed against escherichia coli and staphylococcus aureus. the antibacterial activity was determined by evaluation of minimum inhibitory concentration (mic) and minimum bactericidal concentration (mbc) by broth dilution method in 96-well microplates. the synergistic effects were tested by the checkerboard method and the fractional inhibitory concentration (fic) index was calculated. results: the major components of o. basilicum, s. officinalis and t. ammi were linalool (35.99%), 1,8-cineole (22.91%) and p-cymene (35.5%), respectively. in general, all of the essential oils as well as nisin exerted more considerable antibacterial effects against gram-positive bacteria than gram-negative one. the essential oil of t. ammi showed the highest activity against s. aureus with mic (≤0.078 mg/ml) and mbc (≤0.156 mg/ml). the combined application showed synergistic activity against e. coli but no change in activity was observed against s. aureus. the most synergistic effect was observed for the combination of nisin and s. officinalis (fic 0.03). conclusion: it can be concluded that nisin could enhance the antibacterial potential of the essential oils.
منابع مشابه
Chemical composition and antibacterial properties of Ocimum basilicum, Salvia officinalis and Trachyspermum ammi essential oils alone and in combination with nisin
Background and objectives:Plant essential oils are sometimes considered for use as antimicrobial agents in foods and medicines and they could be combined with other antimicrobial agents to strengthen the effect and/or reduce the required dose. This study was conducted to determine the chemical composition of the Ocimum basilicum, Salvia officinalis and Tra...
متن کاملChemical composition and antibacterial properties of Ocimum basilicum, Salvia officinalis and Trachyspermum ammi essential oils alone and in combination with nisin
Background and objectives: Plant essential oils are sometimes considered for use as antimicrobial agents in foods and medicines and they could be combined with other antimicrobial agents to strengthen the effect and/or reduce the required dose. This study was conducted to determine the chemical composition of the Ocimum basilicum, Salvia officinalis and Trachyspermum ammi essential oils and eva...
متن کاملCentral Properties and Chemical Composition of Ocimum basilicum Essential Oil
Ocimum basilicum L. (Lamiaceae) is an Egyptian plant used as a folkloric remedy in Egyptian traditional medicine. In the current study, the aerial part of this plant was used, and its essential oil was obtained by hydrodistillation. The essential oil of Ocimum basilicum (OB) was screened for its composition and some CNS activities (viz., sedative, hypnotic, anticonvulsant, local anesthetic). Wh...
متن کاملChemical composition, antioxidant, antimicrobial and cytotoxic activities of Tagetes minuta and Ocimum basilicum essential oils
Chemical composition, antioxidant, antimicrobial and cytotoxic activities of Tagetes minuta (TM) essential oil (TMO) and Ocimum basilicum (OB) essential oil (OBO) were examined. The main components for TMO were dihydrotagetone (33.9%), E-ocimene (19.9%), tagetone (16.1%), cis-β-ocimene (7.9%), Z-ocimene (5.3%), limonene (3.1%) and epoxyocimene (2.03%). The main components for OBO were methylcha...
متن کاملChemical composition and antimicrobial activity of essential oil of Salvia officinalis L. and Salvia virgata Jacq
Background & Aim:The genus Salvia (commonly known as sage) is a broad genus belonging to the family Lamiaceae. This study is aimed at assessing the essential oil composition and antimicrobial activities of two Salvia species. Experimental: The chemical composition of the essential oils of Salvia officinalis L. and Sa...
متن کاملChemical composition and antimicrobial activity of essential oil of Salvia officinalis L. and Salvia virgata Jacq
Background & Aim:The genus Salvia (commonly known as sage) is a broad genus belonging to the family Lamiaceae. This study is aimed at assessing the essential oil composition and antimicrobial activities of two Salvia species. Experimental: The chemical composition of the essential oils of Salvia officinalis L. and Sa...
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عنوان ژورنال:
research journal of pharmacognosyجلد ۳، شماره ۴، صفحات ۵۱-۵۸
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